Friday, March 30, 2007
Adorable Otter
Pet Food Recall Update

Thursday, March 29, 2007
Lemur Bites Can be Nasty!
No, I didn't get bit by one, but they sure do have a set of chompers! Today we did physicals on two black and white lemurs. What beautiful animals, but those teeth are nasty! The top canines are especially dangerous. The back of those teeth is narrow and almost serrated. It's bad news when they get into a fight, because they can really rip each other up severely. We also had to get blood from a white stork for a preshipment today.+%27Big%27+20070312+07.jpg)
Fish Fryer
Tuesday, March 27, 2007
White Tigers and the Conservation Question
all. Well, a little more story-telling first. Remember Mohan? He was the male white Bengal tiger captured in the 50s. There were reported sightings and even shootings of white tigers before him, but he was the first one caught alive, that we know about. As the story goes, his mother and 2-3 siblings (depending on the account you read) were shot by the Maharajah at the time, but Mohan the Cub, escaped. Fascinated by the idea of catching a white tiger, the Maharajah sent dozens of people out into the jungle to round the cub up. Long story short, he was captured and taken to the Maharajah's palace where he lived out his days in relative captive luxury.
But it certainly wouldn't do to just have one white tiger. More white tiger cubs clearly needed to be created! To do this, the Maharajah bred Mohan to a wild-caught female Bengal of normal coloration, getting a litter of cubs with the typical orange and black look. However, banking on the idea of simple dominant-recessive inheritance of color in tigers (though it hadn't been described as such, yet), the Maharajah then bred Mohan to one of his daughters. Voila: he got some white tiger cubs!
today can be traced back to Mohan and, consequently, all are highly inbred. It is an example of inbreeding, a relatively common practice in the animal world. Closely related animals are bred in the hopes of magnifying and perpetuating a desirable trait. Inbreeding has been used to develop virtually every domestic dog, cat, horse, cow, pig, bird, goat, sheep, rabbit, etc, breed that there is. Unfortunately, while this type of breeding may intensify the desirable traits that are being bred for, it will also magnify the bad traits. Highly inbred animals tend to have a much higher rate of genetically-related deformities and faults. White tigers are especially inbred since there are so few of them and Mohan is essentially the only foundation animal. In the white tiger, common birth defects include everything from crossed eyes to arched backs to a weakened immune system.
So, here is the basic gist of the issue: the white tiger is a man-made phenomenon. The goal of conservation is to maintain species in as close to their natural form as possible and there is a very limited amount of money and resources to do that. Conservationists would like to see all that money go to preserving the various tiger species in their natural form. However, the white tiger is stunning! The white tiger is beautiful! The white tiger is mysterious! People love white tigers! People are much more excited about seeing a white tiger than they are with the "run-of-the-mill" orange and blacks. And, consequently, they are more likely to donate money to an institution that has white tigers. I don't have any actual figures to back it up, but just ask any zoo director or curator. They'll tell you the same story.
tically worthless, messed up creature. Many hardcore conservationists argue that the breeding of tigers for the express purpose of perpetuating the white mutants should be stopped, and the thought certainly has merit. On the other hand, the funds and interest that the white tigers bring in is certainly nice, and could be applied to other species as well. But does the end justify the means? Is it fair to keep white tigers only because they are a money-maker?Monday, March 26, 2007
Wild Cats and White Tigers
Today we had a very busy morning. Five African Wild Cats out at the Wildlife Safari Park needed their annual physicals and vaccinations. Blood was drawn for routine CBCs, serum chemistry along with additional blood, feces and urine for a nutrition study. Then we looked at a swift fox that had gotten into a tussle with another swift fox. She had a torn ear and some bite marks that needed to be cleaned up. After that was done, she was put on some antibiotics and pain medication for the next few days. Then we had to vaccinate 5 pelicans and a raven against the West Nile virus. So, it was a busy day!And it is high time I talked about yet another broad lesson I’ve learned so, here we go! This will be a long post, so bear with me (if you want to, that is :p)! (link to lesson 1, as a refresher)
Lesson 2: Conservation and Population Medicine (and the revelation that money is not green, but black and white)
There are two distinct forms of medicine within veterinary medical practice: population medicine and individual medicine. In the most basic sense, population medicine deals with animals in production situations (feedlots, cow/calf, swine farms, poultry farms, etc), but it can also deal with companion situations. An example would be vaccinating a pet dog against rabies to protect the dog, but also to reduce the incidence of rabies in the area, thereby making the entire population safer. Individual medicine more classically deals with individual companion animals of all shapes and sizes, but is applicable in production schemes as well. Individual dairy and beef cows, for example, routinely get individualized care for specific conditions from milk fever to bloat. Many veterinarians go into certain areas of practice, because they either like or dislike population medicine. In zoo medicine, I have found that you can’t have one without the other.
There are two main reasons that zoos exist: animal display and animal conservation. Some institutions are more heavily involved in one side or the other, but bo
th have a place. At the HDZ, I’ve found that the split is fairly even. Yes, there are many animals on display, but many of them are a part of intensive conservation programs for their species. And there are many animals that are here but not on display, conservation being the primary reason they are here. One example of that would be the Wyoming Toads. Declared extinct in the 90s, an isolated population was later located and captured. Now there is a growing population of these toads (which, at one time, were the most numerous vertebrate in the state of Wyoming) in a handful of conservation institutions across the country, including the HDZ.All of the endangered and threatened species here have an SSP (Species Survival Plan). These plans look at everything from conservation of the natural habitat of these species to breeding programs that will best maintain a genetically healthy population in the future. The SSP is one of the main cornerstones on which conservation stands. It is population medicine on an individual basis.
Now enter the white tiger.
The white tiger is an anomaly in the tiger world; a man-made anomaly. Many have the mistaken notion that this is a unique, highly endangered species of tiger. Others think that all white tigers are actually Siberian tigers, the white coloring helping them to blend into their snowy environment. Both beliefs are actually wrong! In fact, all white tigers alive today can be traced back to one white Bengal tiger, Mohan, who was captured in 1951.Before I continue, I should speak briefly about the inheritance of color in tigers and give you a basic genetics review. The ‘genotype’ of an animal is the genes it has. The ‘phenotype’ is how it expresses those genes; literally what it looks like. The normal color of all species of tigers is the characteristic orange and black that most of us think of first when we think of tigers. The white and black coloring is a simply inherited recessive trait.
Without getting too technical, I’ll use ‘B’ for the dominant color gene (coding for orange and black) and ‘b’ for the recessive color gene. Simple dominant-recessive expression means that an animal will need to be homozygous (having two copies of the same gene) for the recessive gene, or a ‘bb’ genotype, in this example. A tiger that is homozygous dominant (BB) or heterozygous (Bb) will look normal, phenotypically. The Bb tiger, however, may produce a bb white tiger if it is bred to another Bb tiger (or, of course, a bb tiger).Many people are familiar with the good old Punnit square from genetics. The Punnit square is used to predict the ratio of certain genotypes in a given breeding of two parents of a known genotype (or, conversely, to identify the genotypes of the parents, given the phenotypes of the offspring). Using a Punnit square, here are the likely outcomes of tiger breeding for color:
BB x BB = 100% BB offspring = 100% orange/black
BB x Bb = 50% BB + 50% Bb offspring = 100% orange/black
BB x bb = 100% Bb offspring = 100% orange/black
Bb x Bb = 25% BB + 50% Bb + 25% bb offspring = 75% orange/black, 25% white/black
Bb x bb = 50% Bb + 50% bb offspring = 50% orange/black, 50% white/black
bb x bb = 100% bb offspring = 100% white/black
Still with me? Excellent! I’ve gotten quite long today, so you’ll need to tune in tomorrow for what this all has to do with conservation and population medicine!
Saturday, March 24, 2007
Rat Poison in Pet Food
Friday, March 23, 2007
Bundle o' Fur

They certainly didn't make any friends or recruit any more people to their cause on that particular idea!
Thursday, March 22, 2007
More Tigers!

ortunity to dart a few individual tigers several times. One tigress in particular definately hates me! I entered her holding area the other day to dart her (yet again) for the project, and as soon as she saw me, she went balistic. I wish I had been able to film her. It was quite a show! There was much roaring, snarling, charging, and bristling. In fact, she was so frustrated that she couldn't eat me due to the cage, that she started biting the large log in her cage, actually throwing it towards me at one point. Even so, I was able to dart her! 
Pet Food Recall Update

Apparently, the Iowa State University Veterinary Diagnostic Lab has been the main instituition examing tissues from the animals that have dies due to the pet food recall. Here is some text from the website detailing some of their findings:
"Autopsy and microscopic examination of tissues from affected animals indicate acute renal toxicosis including the presence of birefringent crystals, as well as other crystal formations. The effects are consistent with exposure to ethylene glycol and derivatives, mycotoxins, ochratoxin and citrinin, some heavy metals or vitamin D toxicity. At the present time the exact cause of this problem is not yet known. Investigations are continuing."
The full report can be read here. Definately bad news for the animals, their owners and the pet food manufacturer. However, it is great that the ISU pathologists are on top of this national problem!
Tuesday, March 20, 2007
Tiger Project in Full Gear
Today marked a major uptick in our currently running Tiger Project. I'll elaborate a little on what, exactly, is going on. As I mentioned before, the HDZ is one of the leading instituions in research on tiger reproduction. The Tiger Project is part of that research. Last week we immobilized several female tigers and placed hormone implants under their skin. These implants release a hormone known as FSH (Follicle Stimulating Hormone) at a steady rate over a period of several days. As the name implies, this stimulates development of follicles on the ovaries of these cats and will (hopefully) bring them into heat and cycling in a manner so that we can appropriately time other treatments. In this instance, all of the tigresses responded to the FSH, showing strong signs of coming into heat as was expected and hoped for. This week (7 days after the implants were placed), the tigresses are being darted with additional hormones: GNRH (gonadotropin releasing hormone) and hCG (human chorionic gonadotropin) which will stimulate final development, materation and (untimately) ovulation. However, for the Tiger Project, we want to anesthetized these tigresses before they ovulate.
technologies can be used. The simplified version is that we collected eggs from this tiger (got 11 overall), several of which were then injected into her own Fallopian tube along with sperm that we collected from another Siberian tiger this morning in the hopes of getting some cubs! Also, some of these eggs will be transferred into other tigers so that those techniques can be standardized, also to get cubs.
"Big deal," you say. "Why don't you just let them breed naturally or just artificially inseminate them?" Well, there are a few reasons why. First of all, the goal of this research is to improve reproductive techniques in tigers so that natural breeding is not needed to ensure cubs. In order to maintain a healthy population of any species, it is important to maintain a diverse gene pool. For natural breeding, that would mean the nessescity of transporting male and female tigers all over the place so that they could be bred to unique individuals. By perfecting these techniques, we will be able to avoid that very time-consumming (minimal quarentine protocols and time to introduce animals takes literally months) and costly propositition. And as far as artificial insemination goes, it is historically extremely difficult in feline species, especially tigers. These other techniques are actually much more effective at producing cubs.
Pictured at right is a laproscopic view of a tiger ovary with severeal very nice follicles. They appear as "blister-like" vesicles on the surface of the ovary. A needle is inserted into the follicles and the contents are litereally sucked out and collected. Then our reproductiove technologists exam the contents for eggs and grade their developement. Since we were putting several back into the tigress today, the opening of her Fallopian tube was then located and sperm and several mature eggs were put back in. This procedure is called gamete intrafallopian transfer or GIFT. Another method that we will be doing later this week you may have seen video of: where sperm is injected directly into the egg. This method is called intracytoplasmic sperm injection or ICSI. In-vitro fertilization (IVF or "test tube fertilization") has been done several times successfully here at the HDZ, but GIFT and ICSI show promise of higher success rates.
Monday, March 19, 2007
Pet Food Recall

Sunday, March 18, 2007
Polar Bear Fun
No blog-worth procedures for today or yesterday, but I got some great footage of the polar bears playing. They were definitely having a fun time, and were a very popular stop for the guests. The two bears (19 year old sisters, incidentally) would take turns playing with the ball.
There were some minor fights with much growling, roaring and posturing as one decided that she was ready to play with the ball again before the other was finished. More often than not, one bear would play around in the water with the ball for awhile, then either leave the ball in the water or push it up onto land and leave it. Then she would go and start rubbing the water out of her fur while the other would take the ball.Saturday, March 17, 2007
Fantastic Fossa Facts
I wasn't able to get onto the computer yesterday, so I'll give you a morning update. The biggest event of yesterday was the immobilization of a fossa (pronounced FOO-sa). And the biggest part of that was that I got to do the whole procedure! Dr. Armstrong was there for advice and guidance, but I got to come up with the drug dosages, put the dart together, blow dart the fossa, do the whole physical exam, clean his teeth and ears
and then give the reversal agent. The whole procedure went very well, which I was especially relieved about since this particular animal had had some difficult immobilizations in the past. It was great practice, because I had to tweak the drug dosages based what had been done before and find something that would work better for him. I ended up increasing everything a bit, and it worked great! Almost a little too great! He was lateral in 4 minutes after the dart (normal time for my combo should be around 10 minutes), which we thought meant either (1) I went with too high a dosage, or (2)
part of the dose went IV (intravenous) instead of all IM (in muscle). Neither is a big problem, but both need to be recognised so that -- if a problem does come up -- it can be dealt with. The fossa was still a bit reactive, so it took the expected amount of time before "hands on." (picture at left from http://www.shoarns.com/) Judging from the depth, quality, and duration of his anesthesia, Dr. Armstrong was fairly certain by the end that no more than a small amount of drug had gone IV (if any) and the drug dose was appropriate for him.
They are the largest mammalian carnivore on Madagascar, and hunt everything from insects to amphibians to lemurs. (picture at right from www.belfastzoo.co.uk/fossa.asp) Cat-like in appearance and movement, they are amazingly good climbers that can hunt lemurs through the treetops of the rainforest. They can be aggressive towards conspecifics (their own species), so they are typically kept in solitary exhibits. Males are typically slightly larger than females. The fossa that I immobilized was around 20 lbs.
They have an very long, cat-like tail for balancing as they run around in the trees. They are very elusive in the wild and are thought to be mostly nocturnal. (picture at left from http://photos.signonsandiego.com/). Though they do have all of these cat-like attributes about them, they are most closely related to mongooses. Fossa are considered Endangered with an estimated population of less than 2500 in the wild. The biggest problem is that the remaining animals are scattered in isolated patches of forest throughout the island which has been separated by deforestation. Then of course, there is the problem that these opportunistic hunters cannot resist a few domestic chickens now and again, which certainly doesn't endear them to Malagasy farmers. 

Thursday, March 15, 2007
Pumas and Peacocks
The Tiger Project continues to go well. Three more tigers were anesthetized as part of it on Tuesday and one additional tiger on Wednesday. The purpose of the immobilizations this week was to give the tigresses a hormone treatment (I can go further in depth for interested parties!) in preparation for next week when we will be doing artificial insemination (AI), oocyte collection and embryo transfer with these cats. We will definitely have a busy week of it next week! Tigers are the only thing on the schedule, though (inevitably with a collection this large) we will certainly have other creatures to look at as well.
Pictured at right is a Tiger Quoll (Dasyurus maculatus), a very interesting Australian marsupial that looks something like a spotted opossum with a large, bushy tail. He had a skin infection that needed to be looked at. We also looked at a round ray (I don't recall the species) that had a minor abrasion on her dorsum, a degu that was feeling under the weather, a bison that was losing weight.
I also
drew blood from the peacock at left for routine testing before he is taken to another zoo and from the puma at right. And below is a picture of "Boop," one of 3 Blue Monkeys that had their routine physical exams and vaccinations done.
We also took a look at this Pronghorn antelope buck named "Goliath." (Here checking out Dr. Napier) About a year ago, he had a very severe fracture of his right humerus that required extensive surgery to fix. He is currently doing very well, except he moves gingerly when he gets up after lying down for awhile. He is definitely a favorite among the staff, and quite a nice buck.
And to further expand on my previous post about the lemur species that have been discovered here, the picture at right shows a wall where they have the pictures of all of the species of Sportive Lemurs that were discovered here in 2006 alone. And pictured at right is one of the mouse lemurs.
Many of these species have been differentiated from other species primarily through DNA analysis.
Well, that's about enough for today. I'll continue with more "Lessons" on any upcoming days when I don't have as much to report. If any readers have any special requests regarding information/pictures and/or discussion of certain species, procedures, or topics, please let me know!
New Leopard Species in Borneo
In the meantime, here is an interesting report about a new clouded leopard species that has been identifyed in the rainforests of Borneo. It's amazing to me that new species are still being discovered at the rate that they are! Here at the HDZ, they have identified a dozen new species of mouse lemurs from Madagascar in the last year alone! These species include the Simmons Mouse Lemur, among others.
Monday, March 12, 2007
Tiger Project (Day 1)
Today we started "The Tiger Project" as it is called around here. The HDZ is nationally renowned for their great successes in breeding tigers. Much of that success is thanks to the years of dedicated research that has gone into tiger reproduction. The Project is a continuation of that research. Over the course of this week, we will be immobilizing several of the female tigers as part of a hormone study and in preparation for further reproductive work with them this spring. During these immobilizations, the tigers are also given a complete physical, a teeth cleaning (if needed), any needed vaccinations, and blood collecting for routine analysis and banking. The tiger at left is just starting to wake up after everything was finished. That was about all the blog-worthy stuff we did for today!
Some, like the tiger to the right, simply try to hide. Others are not so subtle. A cat that is perfectly calm and content may suddenly jump up when they see that vet and charge, hackles raised, teeth barred and roar echoing. The apes, particularly the silverback gorillas, very commonly will show their "toughness" by rushing the front of the exhibit and banging on the glass with their fists. This can be quite startling, particularly to guests that are not expecting it. Once I was walking through the gorilla house with one of the vets. We were walking fairly fast as we just wanted to check on the progress of one of the gorillas, then leave for the next thing on the schedule. Well, one of the big silverbacks saw us walk by in the hall. He immediately jumped up and rushed the glass, completely terrifying the guest who had been watching the previously calm and apparently docile animal. She backed up so fast she crashed right into us! It gets to the point that, in order to not overly stress the animals, the vets don't walk out past the exhibits unless they are dressed in very different clothing than the animals are used to seeing them in. So, if you want zoo animals to like you, become a keeper. They won't like you if you are the vet, no matter how much you like them!



